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Nikon epifluorescence nikon tie inverted microscope
Comparison of UnaG expression by rSA11/NSP3-FL-UnaG versus rSA11/NSP1-FL-UnaG. (A) Images taken at 7.5 h p.i. with an <t>epifluorescence</t> Nikon TiE inverted microscope with a 20x Plan Apo (NA 0.75) differential interference contrast (DIC) objective. (B) Quantification of percent change in UnaG fluorescence (ΔF) in MA104 cells infected with rSA11/ NSP1-FL-UnaG or rSA11/NSP3-FL-UnaG. Single cell analysis (N=270 cells each) was performed to determine the percent ΔF from baseline. Data shown as mean ± standard deviation. ****p <0.0001
Epifluorescence Nikon Tie Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparison of UnaG expression by rSA11/NSP3-FL-UnaG versus rSA11/NSP1-FL-UnaG. (A) Images taken at 7.5 h p.i. with an epifluorescence Nikon TiE inverted microscope with a 20x Plan Apo (NA 0.75) differential interference contrast (DIC) objective. (B) Quantification of percent change in UnaG fluorescence (ΔF) in MA104 cells infected with rSA11/ NSP1-FL-UnaG or rSA11/NSP3-FL-UnaG. Single cell analysis (N=270 cells each) was performed to determine the percent ΔF from baseline. Data shown as mean ± standard deviation. ****p <0.0001

Journal: bioRxiv

Article Title: Generation of Recombinant Rotavirus Expressing NSP3-UnaG Fusion Protein by a Simplified Reverse Genetics System

doi: 10.1101/678292

Figure Lengend Snippet: Comparison of UnaG expression by rSA11/NSP3-FL-UnaG versus rSA11/NSP1-FL-UnaG. (A) Images taken at 7.5 h p.i. with an epifluorescence Nikon TiE inverted microscope with a 20x Plan Apo (NA 0.75) differential interference contrast (DIC) objective. (B) Quantification of percent change in UnaG fluorescence (ΔF) in MA104 cells infected with rSA11/ NSP1-FL-UnaG or rSA11/NSP3-FL-UnaG. Single cell analysis (N=270 cells each) was performed to determine the percent ΔF from baseline. Data shown as mean ± standard deviation. ****p <0.0001

Article Snippet: Images were collected at 6 min intervals from 0 to 16 h p.i. using an epifluorescence Nikon TiE inverted microscope with a 20x Plan Apo (NA 0.75) differential interference contrast (DIC) objective.

Techniques: Expressing, Inverted Microscopy, Fluorescence, Infection, Single-cell Analysis, Standard Deviation